Therefore, experiments were conducted to assess the effect of water stress, pH level, and light requirement on seed germination, and the effect of seed burial depth on seedling emergence. Makes an excellent display in pots, window boxes, as a border or for indoor decoration. Then 2, 5, 10, 15, and 20 mm depths were also marked on each pot with the help of a measuring ruler. After the tenth day the aluminum foil was removed, and the Petri dishes were placed back in the chamber to assess seed viability and most of the seeds germinated within four days. Susan Patterson is a health and gardening advocate. When ageratum plants are well grown and all risk of frost has passed, acclimatise them to outdoor conditions over 7 to 10 days. In agricultural lands, soil disturbance in the form of light tillage or cultivation would probably reduce its germination ability. The Petri dishes were then sealed with parafilm and placed in the germination chamber with the settings described earlier. Discover a faster, simpler path to publishing in a high-quality journal. Yes For example, seeds of Malva neglecta can remain viable for over 100 years [35]. Such emergence patterns have been reported in several Asteraceae weed species such as C. canadensis [12]. This low, mounding plant produces button-like flowers in shades of white, pink and blue. As discussed earlier, A. houstonianum is photoblastic and needs light for germination. Yes The Petri dishes were then sealed with parafilm and placed in the seed germination chamber with the temperature and light set as described above. The first and second round of the experiments were conducted in April/May 2018 and June/July 2018, respectively. Ideal temp. One page of facts is devoted to each seed. The authors confirm that no permission was needed to collect the weed seeds, as well as that the studies did not involve endangered or protected species. We took the Safe Seed Pledge so you don't have to worry about the health of your family.wers look soft and fuzzy. Put seeds no closer than 1/2-inch apart in a larger container or one seed per peat pellet. The ecological significance attributed to the light response in these species is that light acts as a soil depth “indicator,” allowing greater germination of surface seeds than seeds buried in soil [21]. Invasive species are non-native and can include plants, animals and microbes that have been relocated deliberately or accidentally as a result of economic activities [1]. The light intensity was measured at five locations in the chamber and the average was 1.4 W m-2. No seedlings emerged from the seeds that were buried more than 2mm deep. Do not exclude light or allow to dry out. Department of Botany, Tribhuvan University, Amrit Campus, Kathmandu, Nepal, Roles Care & Maintenance: Ageratum Sow indoors. Supervision, The Petri dishes were then sealed with parafilm and placed in the seed germination chamber set at a constant temperature of 20 ± 2°C. Yuan and Wen [9], as mentioned above, conducted their study on A. conyzoides with a 12 h photoperiod of 25 μmol m-2 s-1. Seed germination was evaluated every day until germination ceased. Petri dishes were lined with Whatman No. Seed germination in the distilled water treatment (0 MPa) was considered 100% and germination under the other water potential treatments were expressed as relative to the 0 MPa treatment. Surface sow onto moist well-drained seed compost which has been firmed. Ten ml distilled water were added to each Petri dish with a pipette. Ten ml distilled water was added to each Petri dish with a pipette. Ageratum houstonianumflossflower,bluemink,blueweed,pussy foot,Mexican paintbrush is a coolseasonannual plantoften grown asbeddingin gardens. Germination was counted when the coty- . Each experimental unit was a 15 cm tall plastic pot, filled with soil (sandy loam) collected locally from the NARC experimental farm. However, the longevity of the seeds in the seedbank needs to be determined. Yes Funding: The authors received no specific funding for this work. Start with high-quality seed from a reputable dealer. Again, unlike in the moisture stress study, actual data on germination is presented and not normalized to any of the burial depths. For peat pellets, pour warm water on them according to directions and wait for them to expand. Results indicated that this species was moderately drought-tolerant because germination ceased beyond 0.51 MPa. 50 seeds PLANTING Ageratum houstorianum Annual Germination: 5-10 days Germination Temperature: Optimum soil temperatures 70-75ºF Seed Planting Depth: Sow on the surface and press into soil, seeds need light to germinate Starting Indoors: Start indoors 6-8 weeks before the last frost. For example, a study evaluated the effect of temperature and water stress on seed germination of A. conyzoides [9]. However, it is not known what level of light intensity was required for germination as the results of the study is limited to the light setting in the germination chamber. Sow from 4 weeks prior to the last frost in spring under cover with warmth provided. The seeds were tested for germination on, a) the day they were harvested, b) one week after they were harvested, c) two weeks after they were harvested, d) three weeks after they were harvested, and e) four weeks after they harvested. Echinochloa colona seeds germinated up to -5.5 MPa [14]. Examples include Conyza canadensis [12] and Asclepias syriaca L. [20]. The experiment was arranged as a completely randomized design with eight replications and conducted twice. here. 15] including some Asteraceae species [12, 19]. In our study, germination of A. houstonianum seeds occurred between a pH range of 6 and 9 with maximum germination at pH 7. broad scope, and wide readership – a perfect fit for your research every time. Keep soil damp but not wet. Keep planting media moist during the germination period. Use warm water for irrigation. These results suggested that light was a requirement for seed germination in this species. where y is the response variable; a is the upper asymptote (maximum); x is the fixed variable; x0 is the water potential or seed burial depth resulting in 50% value of y; and b is the slope of the curve at x0. Our findings are similar to reports for several other weed species that lack a light trigger for germination [29]. When the seeds that failed to germinate in the various treatments were washed with distilled water and placed back in the germination chamber in petri dishes with new filter paper, approximately 80% of the seeds exposed to various water potentials germinated, indicating that the seeds were still viable. It’s easy to maintain seeds in that range under a fluorescent lamp because it naturally gives off a gentle warmth in the right range. The first and second round of the experiments were conducted in April/May 2018 and June/July 2018, respectively. Data that failed to meet the assumptions of normality and were transformed using log10 procedure prior to analysis (data for the pH experiment did not need transformation). The seeds that were not tested for germination the day that they were collected were stored under room conditions as described earlier, till their designated day of treatment. Once a seed had germinated, it was counted and removed from the Petri dish using tweezers. However, evaluation was continued up to ten days to assess the germination of the seeds under the dark treatment. Since the light level in the germination chamber was quite low (approximately 1.4 W/m2), it can be suggested that seed germination in this species may not require very high light intensities, but some level of light is required. The regression line … Cultivation Advice Ageratum Blue Mink. SOWING: Transplant (recommended): Sow 6-8 weeks before the last frost. Citation: Lamsal A, Devkota MP, Shrestha DS, Joshi S, Shrestha A (2019) Seed germination ecology of Ageratum houstonianum: A major invasive weed in Nepal. No seeds that were buried more than 2 mm deep in the soil emerged. Any seed that failed to germinate in each treatment was collected carefully with a tweezer, washed in distilled water, transferred to a Petri dish lined with a filter paper, 10 ml of distilled water was added, and the Petri dish was placed back into the chamber. 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